MTT Assay:Article Title: Brown adipose tissue involution associated with progressive restriction in progenitor competence.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase I (for mouse and rabbit samples) Worthington Cat#LS004196 Collagenase I (for human sample) Diomond Cat#A004194 Liver Digest Medium Thermo Fisher Scientific Cat# 17703-034 40um Filter Fisher Brand Cat#22363549 ACK Buffer (for mouse and rabbit sample) Quality Biological Cat#118-156-101 ACK Buffer (For human sample) Sangon Biotech Cat#B541001 Ciprofloxacin HCl Sigma-Aldrich Cat#PHR1044 Blasticidin InvivoGen Cat#ant-bl Primocin InvivoGen Cat#ant-pm-1 MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide) Sigma-Aldrich Cat#M2128 PEI Max Polysciences Cat# 24765-1 Insulin (Novolin R ) Novo Nordisk Cat# 0169-1833-11 T3 (3,30,5-Triiodo-L-thyronine sodium salt) Sigma-Aldrich Cat#T6397 IBMX (3-Isobutyl-1-methylxanthine) Sigma-Aldrich Cat#I5879 Rosiglitazone Sigma-Aldrich Cat#R2408 Dexamethasone Sigma-Aldrich Cat#D4902 Indomethacin Sigma-Aldrich Cat#I7378 Biotin Sigma-Aldrich Cat# B4639 Transferrin Sigma-Aldrich Cat# T8158 D-Pantothenic Acid RPI Cat#P55650 Primocin InvivoGen Cat#ant-pm-1 2-Deoxy-D-Glucose (2-DG) Sigma-Aldrich Cat#D6134 Mirabegron XA BC-Biotech N/A CL316,243 Tocris Cat#1499 Basal DMEM Sigma-Aldrich Cat#D5030 GlutaMaxTM Thermo Fisher Scientific Cat#13462629 Fatty acid-free BAS Sigma-Aldrich Cat#A3803 Oligomycin Sigma-Aldrich Cat#O4876 Antimycin Sigma-Aldrich Cat#A8674 Norepinephrine Sigma-Aldrich Cat#A9512 TRIzol Thermo Fisher Scientific Cat# 15596026 Matrigel Coring Cat#356237 Isoflurane Piramal Cat#66704-013-025 DMSO Sigma-Aldrich Cat#D4540 KolliphorEL Sigma-Aldrich Cat#C5135 Protein A/G Santa Cruz Biotechnology Cat#SC-2027 Formalin solution Sigma-Aldrich Cat#HT501128 VECTASHIELD Antifade Mounting Medium with DAPI Vector Cat#H-1200 Target Retrieval Solution, Citrate pH 6.1 Agilent Cat#S1699 Critical commercial assays Dead cell removal kit Miltenyi Biotec Cat#130-090-101 Fixable Viability Dye eFluorTM 780 eBioscience 65-0865-14 PureLinkTM RNA Mini Kit Thermo Fisher Scientific Cat#12183018A iScriptTM cDNA Synthesis Kit Bio-Rad Cat#1708891 iTaqTM Universal SYBR Green Supermix Bio-Rad Cat#172-5125 Histostain -Plus 3rd Gen IHC Detection Kit Thermo Fisher Scientific Cat#85-9073 (Continued on next page) e2 Cell Reports 39, 110575, April 12, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-seq of iBAT from Myf5-Fslt1 KO mice This paper GSE148888 Bluk and scRNA-seq of rabbit BAT This paper GSE148891 Experimental models: Cell lines hTERT A41hBAT-SVF ATCC CRL-3385 hTERT A41hWAT-SVF ATCC CRL-3386 Human fetal BAT SVF Xue et al., 2015 N/A Mouse iBAT SVF This paper N/A Rabbit iBAT SVF This paper N/A 293FT Thermo Fisher Scientific Cat#R70007 Experimental models: Organisms/strains Fstl1fl/fl: B6-Fstl1f3,4/+/Nju National Resource Center for Mutant Mice of China Stock No: B000172 Ucp1-Cre: B6.FVB-Tg(Ucp1-cre)1Evdr/J The Jackson Laboratory Stock No: 024670 Myf5-Cre: B6.129S4-Myf5tm3(cre)Sor/J The Jackson Laboratory Stock No: 007893 Adipoq-Cre: B6;FVB-Tg(Adipoq-cre)1Evdr/J The Jackson Laboratory Stock No: 010803 HSA-Cre: B6.Cg-Tg(ACTA1-cre)79Jme/J The Jackson Laboratory Stock No: 006149 Fstl1-CreER: B6N;129-Fstl1tm1(CreERT2)/Nju National Resource Center for Mutant Mice of China N/A Rosa26-mTmG: B6.129(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo/J The Jackson Laboratory Stock No: 007676 Rosa26-DTR:C57BL/6-Gt(ROSA) 26Sortm1(HBEGF)Awai/J The Jackson Laboratory Stock No: 007900 NCG: NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl Charles River 572 New Zealand White Rabbits Bakkom Rabbitry N/A Oligonucleotides Table S11 This paper N/A Recombinant DNA Plasmid: pLenti-SV40T-P2a-Blasticidin This paper N/A Plasmid: pLXSN2-Wnt10b-HA (NM_011718.2) Kang et al., 2005 N/A Plasmid: pLVX-FSTL1-Myc WT(NM_008047.5) This paper N/A Plasmid: pLVX-FSTL1-Myc MT(N142Q;N173Q;N178Q) This paper N/A Plasmid: pSPAX2 Addgene #12260 Plasmid: pMD2G Addgene #12259 Plasmid: pLVX-Dsred-MonoN1 Clontech Cat#632152 Plasmid: pLVX-Wnt10b (NM_011718.2) This paper N/A Software and algorithms FLIR Tools 5.13 https://www.flir.com/products/flir-tools/ Excel v16.16.21 https://www.microsoft.com Graphpad v7.0 https://www.graphpad.com FlowJo v10 https://www.flowjo.com SOAPnuke v1.5.2 https://github.com/BGI-flexlab/SOAPnuke HISAT2 v2.0.4 http://daehwankimlab.github.io/hisat2/ Bowtie2 v2.2.5 http://bowtie-bio.sourceforge.net/bowtie2 RSEM v1.2.12 http://deweylab.biostat.wisc.edu/RSEM DEseq2 v1.26.0 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html Cell Ranger Single Cell Software Suite v3.1.0 https://www.10xgenomics.com Seurat v3.1.5 https://satijalab.org/seurat/ Cell Reports 39, 110575, April 12, 2022 e3
cDNA Synthesis:Article Title: Brown adipose tissue involution associated with progressive restriction in progenitor competence.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase I (for mouse and rabbit samples) Worthington Cat#LS004196 Collagenase I (for human sample) Diomond Cat#A004194 Liver Digest Medium Thermo Fisher Scientific Cat# 17703-034 40um Filter Fisher Brand Cat#22363549 ACK Buffer (for mouse and rabbit sample) Quality Biological Cat#118-156-101 ACK Buffer (For human sample) Sangon Biotech Cat#B541001 Ciprofloxacin HCl Sigma-Aldrich Cat#PHR1044 Blasticidin InvivoGen Cat#ant-bl Primocin InvivoGen Cat#ant-pm-1 MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide) Sigma-Aldrich Cat#M2128 PEI Max Polysciences Cat# 24765-1 Insulin (Novolin R ) Novo Nordisk Cat# 0169-1833-11 T3 (3,30,5-Triiodo-L-thyronine sodium salt) Sigma-Aldrich Cat#T6397 IBMX (3-Isobutyl-1-methylxanthine) Sigma-Aldrich Cat#I5879 Rosiglitazone Sigma-Aldrich Cat#R2408 Dexamethasone Sigma-Aldrich Cat#D4902 Indomethacin Sigma-Aldrich Cat#I7378 Biotin Sigma-Aldrich Cat# B4639 Transferrin Sigma-Aldrich Cat# T8158 D-Pantothenic Acid RPI Cat#P55650 Primocin InvivoGen Cat#ant-pm-1 2-Deoxy-D-Glucose (2-DG) Sigma-Aldrich Cat#D6134 Mirabegron XA BC-Biotech N/A CL316,243 Tocris Cat#1499 Basal DMEM Sigma-Aldrich Cat#D5030 GlutaMaxTM Thermo Fisher Scientific Cat#13462629 Fatty acid-free BAS Sigma-Aldrich Cat#A3803 Oligomycin Sigma-Aldrich Cat#O4876 Antimycin Sigma-Aldrich Cat#A8674 Norepinephrine Sigma-Aldrich Cat#A9512 TRIzol Thermo Fisher Scientific Cat# 15596026 Matrigel Coring Cat#356237 Isoflurane Piramal Cat#66704-013-025 DMSO Sigma-Aldrich Cat#D4540 KolliphorEL Sigma-Aldrich Cat#C5135 Protein A/G Santa Cruz Biotechnology Cat#SC-2027 Formalin solution Sigma-Aldrich Cat#HT501128 VECTASHIELD Antifade Mounting Medium with DAPI Vector Cat#H-1200 Target Retrieval Solution, Citrate pH 6.1 Agilent Cat#S1699 Critical commercial assays Dead cell removal kit Miltenyi Biotec Cat#130-090-101 Fixable Viability Dye eFluorTM 780 eBioscience 65-0865-14 PureLinkTM RNA Mini Kit Thermo Fisher Scientific Cat#12183018A iScriptTM cDNA Synthesis Kit Bio-Rad Cat#1708891 iTaqTM Universal SYBR Green Supermix Bio-Rad Cat#172-5125 Histostain -Plus 3rd Gen IHC Detection Kit Thermo Fisher Scientific Cat#85-9073 (Continued on next page) e2 Cell Reports 39, 110575, April 12, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-seq of iBAT from Myf5-Fslt1 KO mice This paper GSE148888 Bluk and scRNA-seq of rabbit BAT This paper GSE148891 Experimental models: Cell lines hTERT A41hBAT-SVF ATCC CRL-3385 hTERT A41hWAT-SVF ATCC CRL-3386 Human fetal BAT SVF Xue et al., 2015 N/A Mouse iBAT SVF This paper N/A Rabbit iBAT SVF This paper N/A 293FT Thermo Fisher Scientific Cat#R70007 Experimental models: Organisms/strains Fstl1fl/fl: B6-Fstl1f3,4/+/Nju National Resource Center for Mutant Mice of China Stock No: B000172 Ucp1-Cre: B6.FVB-Tg(Ucp1-cre)1Evdr/J The Jackson Laboratory Stock No: 024670 Myf5-Cre: B6.129S4-Myf5tm3(cre)Sor/J The Jackson Laboratory Stock No: 007893 Adipoq-Cre: B6;FVB-Tg(Adipoq-cre)1Evdr/J The Jackson Laboratory Stock No: 010803 HSA-Cre: B6.Cg-Tg(ACTA1-cre)79Jme/J The Jackson Laboratory Stock No: 006149 Fstl1-CreER: B6N;129-Fstl1tm1(CreERT2)/Nju National Resource Center for Mutant Mice of China N/A Rosa26-mTmG: B6.129(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo/J The Jackson Laboratory Stock No: 007676 Rosa26-DTR:C57BL/6-Gt(ROSA) 26Sortm1(HBEGF)Awai/J The Jackson Laboratory Stock No: 007900 NCG: NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl Charles River 572 New Zealand White Rabbits Bakkom Rabbitry N/A Oligonucleotides Table S11 This paper N/A Recombinant DNA Plasmid: pLenti-SV40T-P2a-Blasticidin This paper N/A Plasmid: pLXSN2-Wnt10b-HA (NM_011718.2) Kang et al., 2005 N/A Plasmid: pLVX-FSTL1-Myc WT(NM_008047.5) This paper N/A Plasmid: pLVX-FSTL1-Myc MT(N142Q;N173Q;N178Q) This paper N/A Plasmid: pSPAX2 Addgene #12260 Plasmid: pMD2G Addgene #12259 Plasmid: pLVX-Dsred-MonoN1 Clontech Cat#632152 Plasmid: pLVX-Wnt10b (NM_011718.2) This paper N/A Software and algorithms FLIR Tools 5.13 https://www.flir.com/products/flir-tools/ Excel v16.16.21 https://www.microsoft.com Graphpad v7.0 https://www.graphpad.com FlowJo v10 https://www.flowjo.com SOAPnuke v1.5.2 https://github.com/BGI-flexlab/SOAPnuke HISAT2 v2.0.4 http://daehwankimlab.github.io/hisat2/ Bowtie2 v2.2.5 http://bowtie-bio.sourceforge.net/bowtie2 RSEM v1.2.12 http://deweylab.biostat.wisc.edu/RSEM DEseq2 v1.26.0 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html Cell Ranger Single Cell Software Suite v3.1.0 https://www.10xgenomics.com Seurat v3.1.5 https://satijalab.org/seurat/ Cell Reports 39, 110575, April 12, 2022 e3
SYBR Green Assay:Article Title: Brown adipose tissue involution associated with progressive restriction in progenitor competence.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase I (for mouse and rabbit samples) Worthington Cat#LS004196 Collagenase I (for human sample) Diomond Cat#A004194 Liver Digest Medium Thermo Fisher Scientific Cat# 17703-034 40um Filter Fisher Brand Cat#22363549 ACK Buffer (for mouse and rabbit sample) Quality Biological Cat#118-156-101 ACK Buffer (For human sample) Sangon Biotech Cat#B541001 Ciprofloxacin HCl Sigma-Aldrich Cat#PHR1044 Blasticidin InvivoGen Cat#ant-bl Primocin InvivoGen Cat#ant-pm-1 MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide) Sigma-Aldrich Cat#M2128 PEI Max Polysciences Cat# 24765-1 Insulin (Novolin R ) Novo Nordisk Cat# 0169-1833-11 T3 (3,30,5-Triiodo-L-thyronine sodium salt) Sigma-Aldrich Cat#T6397 IBMX (3-Isobutyl-1-methylxanthine) Sigma-Aldrich Cat#I5879 Rosiglitazone Sigma-Aldrich Cat#R2408 Dexamethasone Sigma-Aldrich Cat#D4902 Indomethacin Sigma-Aldrich Cat#I7378 Biotin Sigma-Aldrich Cat# B4639 Transferrin Sigma-Aldrich Cat# T8158 D-Pantothenic Acid RPI Cat#P55650 Primocin InvivoGen Cat#ant-pm-1 2-Deoxy-D-Glucose (2-DG) Sigma-Aldrich Cat#D6134 Mirabegron XA BC-Biotech N/A CL316,243 Tocris Cat#1499 Basal DMEM Sigma-Aldrich Cat#D5030 GlutaMaxTM Thermo Fisher Scientific Cat#13462629 Fatty acid-free BAS Sigma-Aldrich Cat#A3803 Oligomycin Sigma-Aldrich Cat#O4876 Antimycin Sigma-Aldrich Cat#A8674 Norepinephrine Sigma-Aldrich Cat#A9512 TRIzol Thermo Fisher Scientific Cat# 15596026 Matrigel Coring Cat#356237 Isoflurane Piramal Cat#66704-013-025 DMSO Sigma-Aldrich Cat#D4540 KolliphorEL Sigma-Aldrich Cat#C5135 Protein A/G Santa Cruz Biotechnology Cat#SC-2027 Formalin solution Sigma-Aldrich Cat#HT501128 VECTASHIELD Antifade Mounting Medium with DAPI Vector Cat#H-1200 Target Retrieval Solution, Citrate pH 6.1 Agilent Cat#S1699 Critical commercial assays Dead cell removal kit Miltenyi Biotec Cat#130-090-101 Fixable Viability Dye eFluorTM 780 eBioscience 65-0865-14 PureLinkTM RNA Mini Kit Thermo Fisher Scientific Cat#12183018A iScriptTM cDNA Synthesis Kit Bio-Rad Cat#1708891 iTaqTM Universal SYBR Green Supermix Bio-Rad Cat#172-5125 Histostain -Plus 3rd Gen IHC Detection Kit Thermo Fisher Scientific Cat#85-9073 (Continued on next page) e2 Cell Reports 39, 110575, April 12, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-seq of iBAT from Myf5-Fslt1 KO mice This paper GSE148888 Bluk and scRNA-seq of rabbit BAT This paper GSE148891 Experimental models: Cell lines hTERT A41hBAT-SVF ATCC CRL-3385 hTERT A41hWAT-SVF ATCC CRL-3386 Human fetal BAT SVF Xue et al., 2015 N/A Mouse iBAT SVF This paper N/A Rabbit iBAT SVF This paper N/A 293FT Thermo Fisher Scientific Cat#R70007 Experimental models: Organisms/strains Fstl1fl/fl: B6-Fstl1f3,4/+/Nju National Resource Center for Mutant Mice of China Stock No: B000172 Ucp1-Cre: B6.FVB-Tg(Ucp1-cre)1Evdr/J The Jackson Laboratory Stock No: 024670 Myf5-Cre: B6.129S4-Myf5tm3(cre)Sor/J The Jackson Laboratory Stock No: 007893 Adipoq-Cre: B6;FVB-Tg(Adipoq-cre)1Evdr/J The Jackson Laboratory Stock No: 010803 HSA-Cre: B6.Cg-Tg(ACTA1-cre)79Jme/J The Jackson Laboratory Stock No: 006149 Fstl1-CreER: B6N;129-Fstl1tm1(CreERT2)/Nju National Resource Center for Mutant Mice of China N/A Rosa26-mTmG: B6.129(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo/J The Jackson Laboratory Stock No: 007676 Rosa26-DTR:C57BL/6-Gt(ROSA) 26Sortm1(HBEGF)Awai/J The Jackson Laboratory Stock No: 007900 NCG: NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl Charles River 572 New Zealand White Rabbits Bakkom Rabbitry N/A Oligonucleotides Table S11 This paper N/A Recombinant DNA Plasmid: pLenti-SV40T-P2a-Blasticidin This paper N/A Plasmid: pLXSN2-Wnt10b-HA (NM_011718.2) Kang et al., 2005 N/A Plasmid: pLVX-FSTL1-Myc WT(NM_008047.5) This paper N/A Plasmid: pLVX-FSTL1-Myc MT(N142Q;N173Q;N178Q) This paper N/A Plasmid: pSPAX2 Addgene #12260 Plasmid: pMD2G Addgene #12259 Plasmid: pLVX-Dsred-MonoN1 Clontech Cat#632152 Plasmid: pLVX-Wnt10b (NM_011718.2) This paper N/A Software and algorithms FLIR Tools 5.13 https://www.flir.com/products/flir-tools/ Excel v16.16.21 https://www.microsoft.com Graphpad v7.0 https://www.graphpad.com FlowJo v10 https://www.flowjo.com SOAPnuke v1.5.2 https://github.com/BGI-flexlab/SOAPnuke HISAT2 v2.0.4 http://daehwankimlab.github.io/hisat2/ Bowtie2 v2.2.5 http://bowtie-bio.sourceforge.net/bowtie2 RSEM v1.2.12 http://deweylab.biostat.wisc.edu/RSEM DEseq2 v1.26.0 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html Cell Ranger Single Cell Software Suite v3.1.0 https://www.10xgenomics.com Seurat v3.1.5 https://satijalab.org/seurat/ Cell Reports 39, 110575, April 12, 2022 e3
Immunohistochemistry:Article Title: Brown adipose tissue involution associated with progressive restriction in progenitor competence.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase I (for mouse and rabbit samples) Worthington Cat#LS004196 Collagenase I (for human sample) Diomond Cat#A004194 Liver Digest Medium Thermo Fisher Scientific Cat# 17703-034 40um Filter Fisher Brand Cat#22363549 ACK Buffer (for mouse and rabbit sample) Quality Biological Cat#118-156-101 ACK Buffer (For human sample) Sangon Biotech Cat#B541001 Ciprofloxacin HCl Sigma-Aldrich Cat#PHR1044 Blasticidin InvivoGen Cat#ant-bl Primocin InvivoGen Cat#ant-pm-1 MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide) Sigma-Aldrich Cat#M2128 PEI Max Polysciences Cat# 24765-1 Insulin (Novolin R ) Novo Nordisk Cat# 0169-1833-11 T3 (3,30,5-Triiodo-L-thyronine sodium salt) Sigma-Aldrich Cat#T6397 IBMX (3-Isobutyl-1-methylxanthine) Sigma-Aldrich Cat#I5879 Rosiglitazone Sigma-Aldrich Cat#R2408 Dexamethasone Sigma-Aldrich Cat#D4902 Indomethacin Sigma-Aldrich Cat#I7378 Biotin Sigma-Aldrich Cat# B4639 Transferrin Sigma-Aldrich Cat# T8158 D-Pantothenic Acid RPI Cat#P55650 Primocin InvivoGen Cat#ant-pm-1 2-Deoxy-D-Glucose (2-DG) Sigma-Aldrich Cat#D6134 Mirabegron XA BC-Biotech N/A CL316,243 Tocris Cat#1499 Basal DMEM Sigma-Aldrich Cat#D5030 GlutaMaxTM Thermo Fisher Scientific Cat#13462629 Fatty acid-free BAS Sigma-Aldrich Cat#A3803 Oligomycin Sigma-Aldrich Cat#O4876 Antimycin Sigma-Aldrich Cat#A8674 Norepinephrine Sigma-Aldrich Cat#A9512 TRIzol Thermo Fisher Scientific Cat# 15596026 Matrigel Coring Cat#356237 Isoflurane Piramal Cat#66704-013-025 DMSO Sigma-Aldrich Cat#D4540 KolliphorEL Sigma-Aldrich Cat#C5135 Protein A/G Santa Cruz Biotechnology Cat#SC-2027 Formalin solution Sigma-Aldrich Cat#HT501128 VECTASHIELD Antifade Mounting Medium with DAPI Vector Cat#H-1200 Target Retrieval Solution, Citrate pH 6.1 Agilent Cat#S1699 Critical commercial assays Dead cell removal kit Miltenyi Biotec Cat#130-090-101 Fixable Viability Dye eFluorTM 780 eBioscience 65-0865-14 PureLinkTM RNA Mini Kit Thermo Fisher Scientific Cat#12183018A iScriptTM cDNA Synthesis Kit Bio-Rad Cat#1708891 iTaqTM Universal SYBR Green Supermix Bio-Rad Cat#172-5125 Histostain -Plus 3rd Gen IHC Detection Kit Thermo Fisher Scientific Cat#85-9073 (Continued on next page) e2 Cell Reports 39, 110575, April 12, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-seq of iBAT from Myf5-Fslt1 KO mice This paper GSE148888 Bluk and scRNA-seq of rabbit BAT This paper GSE148891 Experimental models: Cell lines hTERT A41hBAT-SVF ATCC CRL-3385 hTERT A41hWAT-SVF ATCC CRL-3386 Human fetal BAT SVF Xue et al., 2015 N/A Mouse iBAT SVF This paper N/A Rabbit iBAT SVF This paper N/A 293FT Thermo Fisher Scientific Cat#R70007 Experimental models: Organisms/strains Fstl1fl/fl: B6-Fstl1f3,4/+/Nju National Resource Center for Mutant Mice of China Stock No: B000172 Ucp1-Cre: B6.FVB-Tg(Ucp1-cre)1Evdr/J The Jackson Laboratory Stock No: 024670 Myf5-Cre: B6.129S4-Myf5tm3(cre)Sor/J The Jackson Laboratory Stock No: 007893 Adipoq-Cre: B6;FVB-Tg(Adipoq-cre)1Evdr/J The Jackson Laboratory Stock No: 010803 HSA-Cre: B6.Cg-Tg(ACTA1-cre)79Jme/J The Jackson Laboratory Stock No: 006149 Fstl1-CreER: B6N;129-Fstl1tm1(CreERT2)/Nju National Resource Center for Mutant Mice of China N/A Rosa26-mTmG: B6.129(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo/J The Jackson Laboratory Stock No: 007676 Rosa26-DTR:C57BL/6-Gt(ROSA) 26Sortm1(HBEGF)Awai/J The Jackson Laboratory Stock No: 007900 NCG: NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl Charles River 572 New Zealand White Rabbits Bakkom Rabbitry N/A Oligonucleotides Table S11 This paper N/A Recombinant DNA Plasmid: pLenti-SV40T-P2a-Blasticidin This paper N/A Plasmid: pLXSN2-Wnt10b-HA (NM_011718.2) Kang et al., 2005 N/A Plasmid: pLVX-FSTL1-Myc WT(NM_008047.5) This paper N/A Plasmid: pLVX-FSTL1-Myc MT(N142Q;N173Q;N178Q) This paper N/A Plasmid: pSPAX2 Addgene #12260 Plasmid: pMD2G Addgene #12259 Plasmid: pLVX-Dsred-MonoN1 Clontech Cat#632152 Plasmid: pLVX-Wnt10b (NM_011718.2) This paper N/A Software and algorithms FLIR Tools 5.13 https://www.flir.com/products/flir-tools/ Excel v16.16.21 https://www.microsoft.com Graphpad v7.0 https://www.graphpad.com FlowJo v10 https://www.flowjo.com SOAPnuke v1.5.2 https://github.com/BGI-flexlab/SOAPnuke HISAT2 v2.0.4 http://daehwankimlab.github.io/hisat2/ Bowtie2 v2.2.5 http://bowtie-bio.sourceforge.net/bowtie2 RSEM v1.2.12 http://deweylab.biostat.wisc.edu/RSEM DEseq2 v1.26.0 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html Cell Ranger Single Cell Software Suite v3.1.0 https://www.10xgenomics.com Seurat v3.1.5 https://satijalab.org/seurat/ Cell Reports 39, 110575, April 12, 2022 e3
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